nk cell isolation kit rosettesep kit  (STEMCELL Technologies Inc)

Journal: Journal of proteomics

Article Title: Differential expression of proteins in naïve and IL-2 stimulated primary human NK cells identified by global proteomic analysis

doi: 10.1016/j.jprot.2013.06.024

Figure Lengend Snippet: NK cell activation. NK cells isolated from the peripheral blood of healthy donors were stimulated for 16 h with IL-2. Activation status of the NK cells was determined by monitoring increase in the CD69 levels on the surface of the NK cells using flow cytometry. Data shown is for NK cells isolated from two different healthy donors. Control NK cells (dotted line) and IL-2 stimulated cells (solid line) were labeled with FITC-conjugated anti-CD69 antibody. Shaded area depicts binding of non-specific murine IgG antibody.

Article Snippet: The RosetteSep NK cell isolation kit (Stem Cell Technologies) was used and NK cell purification was conducted according to the manufacturer's protocol and the purity of the isolated NK cells was determined by monitoring of CD3, CD16, CD56, and NKp46 expression via flow cytometry as described in our previous work [ 27 – 29 ].

Techniques: Activation Assay, Isolation, Flow Cytometry, Control, Labeling, Binding Assay

Journal: Journal of proteomics

Article Title: Differential expression of proteins in naïve and IL-2 stimulated primary human NK cells identified by global proteomic analysis

doi: 10.1016/j.jprot.2013.06.024

Figure Lengend Snippet: Venn diagram depicting the total number of proteins identified in naïve or IL-2-activated NK cells. Human primary NK cells isolated from three healthy donors were cultured in medium supplemented with or without IL-2 for 16 h. Total proteins were extracted and digested by trypsin follow by 2D LC–MS/MS analysis. 2311 proteins were identified from naïve NK cell samples, while 2413 proteins were identified in IL-2-activated NK cells. 1999 proteins were commonly identified in both conditions.

Article Snippet: The RosetteSep NK cell isolation kit (Stem Cell Technologies) was used and NK cell purification was conducted according to the manufacturer's protocol and the purity of the isolated NK cells was determined by monitoring of CD3, CD16, CD56, and NKp46 expression via flow cytometry as described in our previous work [ 27 – 29 ].

Techniques: Isolation, Cell Culture, Liquid Chromatography with Mass Spectroscopy

Journal: Journal of proteomics

Article Title: Differential expression of proteins in naïve and IL-2 stimulated primary human NK cells identified by global proteomic analysis

doi: 10.1016/j.jprot.2013.06.024

Figure Lengend Snippet: Validation of selected factors differentially expressed in IL-2 stimulated NK cells. Real-time-PCR analysis (a) of mRNA level of PTP1B, PCNA, and CD97 in naïve NK cells and NK cells activated by IL-2 for 16 h. Flow cytometry was used to monitor differential expression of CD48 (b), CD56 (c), CD11b (d), and CD11c (e) on naïve NK cells (dotted line) and NK cells stimulated with IL-2 (solid line) for 16 h. For each factor, data obtained for NK cells isolated from two healthy donors is shown. Non-specific IgG control is (shaded histogram) is shown only for the CD48 data.

Article Snippet: The RosetteSep NK cell isolation kit (Stem Cell Technologies) was used and NK cell purification was conducted according to the manufacturer's protocol and the purity of the isolated NK cells was determined by monitoring of CD3, CD16, CD56, and NKp46 expression via flow cytometry as described in our previous work [ 27 – 29 ].

Techniques: Biomarker Discovery, Real-time Polymerase Chain Reaction, Flow Cytometry, Quantitative Proteomics, Isolation, Control

Journal: Journal of proteomics

Article Title: Differential expression of proteins in naïve and IL-2 stimulated primary human NK cells identified by global proteomic analysis

doi: 10.1016/j.jprot.2013.06.024

Figure Lengend Snippet: Table 1

Article Snippet: The RosetteSep NK cell isolation kit (Stem Cell Technologies) was used and NK cell purification was conducted according to the manufacturer's protocol and the purity of the isolated NK cells was determined by monitoring of CD3, CD16, CD56, and NKp46 expression via flow cytometry as described in our previous work [ 27 – 29 ].

Techniques: